Volume 10, Issue 2 (9-2017)
ijhe 2017, 10(2): 175-186 |
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Naqshband N, Askari Hesni M. Study of agricultural pesticide glyphosate effects on hematological factors and behavioral changes in Cyprinus carpio. ijhe 2017; 10 (2) :175-186
URL: http://ijhe.tums.ac.ir/article-1-5847-en.html
URL: http://ijhe.tums.ac.ir/article-1-5847-en.html
1- Department of Biology, Faculty of Sciences, Payame Noor University, Oshnaviye, Iran
2- Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran , mahesni@gmail.com
2- Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran , mahesni@gmail.com
Abstract: (5098 Views)
Background and Objective: Glyphosate is a non-selective, systemic herbicide that can control most plants, but it has harmful effects on organisms, especially aquatic animals. Static bioassays were made to determine acute toxicity of glyphosate and its effects on haematological parameters and behavior changes of common carp (Cyprinus carpio).
Materials and Methods: Common carp were exposed to different concentrations of glyphosate (5, 10, 20, 30 and 40 mg/L) and a control group for 96 h. After that, blood and plasma factors were measured in blood samples. Mortality rate and fish behavioral were recorded during the experiments. LC50, LOEC, NOEC and MATC were calculated by Probit software according to mortality rate in any treatment.
Results: In the early period of the experiment, fish in toxic media were jumping in water and showed high movements. Then after, the behavior such as breathing in surface, loss of balance, weakness and finally death were observed in the different treatments. LC50 value and 95% confidence limit of glyphosate in 24, 48, 72 and 96 h were 36.09, 30.46, 21.78 and 20.05 mg/L for common carp, respectively. The LOEC, NOEC and MATC were calculated as 6.66, 4.73 and 2.005 mg/L. The Significant changes that were observed in blood factors included RBC, WBC, Hct, Hb and plasma factors such as total protein, glucose, cortisol and other factors when the pesticide concentration was increased (p<0.05).
Conclusion: Results showed that glyphosate had a high effect on common carp and it its physiology and immune system. Haematological parameters may be used as an environmental health diagnostic test and stress indicator for agriculture pesticides in aquatic ecosystems.
Materials and Methods: Common carp were exposed to different concentrations of glyphosate (5, 10, 20, 30 and 40 mg/L) and a control group for 96 h. After that, blood and plasma factors were measured in blood samples. Mortality rate and fish behavioral were recorded during the experiments. LC50, LOEC, NOEC and MATC were calculated by Probit software according to mortality rate in any treatment.
Results: In the early period of the experiment, fish in toxic media were jumping in water and showed high movements. Then after, the behavior such as breathing in surface, loss of balance, weakness and finally death were observed in the different treatments. LC50 value and 95% confidence limit of glyphosate in 24, 48, 72 and 96 h were 36.09, 30.46, 21.78 and 20.05 mg/L for common carp, respectively. The LOEC, NOEC and MATC were calculated as 6.66, 4.73 and 2.005 mg/L. The Significant changes that were observed in blood factors included RBC, WBC, Hct, Hb and plasma factors such as total protein, glucose, cortisol and other factors when the pesticide concentration was increased (p<0.05).
Conclusion: Results showed that glyphosate had a high effect on common carp and it its physiology and immune system. Haematological parameters may be used as an environmental health diagnostic test and stress indicator for agriculture pesticides in aquatic ecosystems.
Type of Study: Research |
Subject:
General
Received: 2017/02/4 | Accepted: 2017/08/7 | Published: 2017/09/20
Received: 2017/02/4 | Accepted: 2017/08/7 | Published: 2017/09/20
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