Volume 18, Issue 1 (5-2025)
ijhe 2025, 18(1): 141-154 |
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Naseh Babakhani1 
, Soheil Sobhanardakani *2 , Borhan Mansouri3 , Mehrdad Cheraghi1 , Bahareh Lorestani1
, Soheil Sobhanardakani *2 , Borhan Mansouri3 , Mehrdad Cheraghi1 , Bahareh Lorestani1
1- Department of the Environment, College of Basic Sciences, Hamedan Branch, Islamic Azad University, Hamedan, Iran
2- Department of the Environment, College of Basic Sciences, Hamedan Branch, Islamic Azad University, Hamedan, Iran ,s_sobhan@iauh.ac.ir
3- Substance Abuse Prevention Research Center, Health Research Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
2- Department of the Environment, College of Basic Sciences, Hamedan Branch, Islamic Azad University, Hamedan, Iran ,
3- Substance Abuse Prevention Research Center, Health Research Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract: (1193 Views)
Background and Objective: Growing concerns exist regarding the impact of nanoparticles on organisms and environmental health. This study aimed to investigate the effects of silver and graphene oxide nanoparticles on the gill and intestinal tissues of guppy fish (Poecilia reticulata) during acute toxicity exposure.
Materials and Methods: The acute toxicity (LC50) for both nanoparticles was calculated over a 96-hour period using Probit software. Histopathological examination of the gill and intestinal tissues exposed to the nanoparticles was performed using the hematoxylin-eosin staining method. A semi-quantitative approach was employed to assess the severity of tissue damage.
Results: The LC50 values for silver nanoparticles, graphene oxide nanoparticles, and their combination were determined to be 2.6 mg/L, 65.2 mg/L, and 1.8 mg/L, respectively. Simultaneous exposure to these nanoparticles exhibited a cumulative acute toxicity effect on fish tissues, with the severity increasing progressively from 24 to 96 hours. Notable injuries included hyperplasia, fusion, and adhesion of secondary lamellae in the gill tissue, as well as villi fusion, structural expansion of villi, increased blood cell count, and erosion in the intestinal tissue of the fish.
Conclusion: Simultaneous exposure to silver and graphene oxide nanoparticles significantly increases the acute toxicity in guppy fish. This study highlights the need for further research to fully understand the implications of nanoparticle exposure across the food chain.
Materials and Methods: The acute toxicity (LC50) for both nanoparticles was calculated over a 96-hour period using Probit software. Histopathological examination of the gill and intestinal tissues exposed to the nanoparticles was performed using the hematoxylin-eosin staining method. A semi-quantitative approach was employed to assess the severity of tissue damage.
Results: The LC50 values for silver nanoparticles, graphene oxide nanoparticles, and their combination were determined to be 2.6 mg/L, 65.2 mg/L, and 1.8 mg/L, respectively. Simultaneous exposure to these nanoparticles exhibited a cumulative acute toxicity effect on fish tissues, with the severity increasing progressively from 24 to 96 hours. Notable injuries included hyperplasia, fusion, and adhesion of secondary lamellae in the gill tissue, as well as villi fusion, structural expansion of villi, increased blood cell count, and erosion in the intestinal tissue of the fish.
Conclusion: Simultaneous exposure to silver and graphene oxide nanoparticles significantly increases the acute toxicity in guppy fish. This study highlights the need for further research to fully understand the implications of nanoparticle exposure across the food chain.
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