Volume 3, Issue 3 (4 2010)                   ijhe 2010, 3(3): 0-0 | Back to browse issues page

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Atabakhsh P, Amin M, Mortazavi H, Yaran M, Akhavan Sepahi A, Nouhi A et al . Identification of Total and Fecal Coliforms and Heterotrophic to Microbiological Method and E.coli O157:H7 to Immunological, and RealTime PCR Methodsin IsfahanWaterTreatment Plant. ijhe. 2010; 3 (3)
URL: http://ijhe.tums.ac.ir/article-1-115-en.html
Abstract:   (10089 Views)

Backgrounds and Objectives:Total and Fecal coliforms (TC and FC), heterotrophic plate count (HPC), were counted by microbiological method and E.coli O157:H7 were detected through immunological and Real time PCRmethods inwater intake and all of units of Isfahanwater treatment plant (IWTP).
Materials and Methods: The microbial profile including TC, FC, and HPC, were monitored and turbidity and total organic carbon were analyzed in 8 locations of water intake, and unit operation and processes of IWTP, including, inlet, sedimentation, ozonation, and filtration and finished water. Immunological method through anti-serum kits and molecular method of RT-PCR were used to detect E.coli O157:H7 in the 8 locations and also the sludge of the sedimentation basin and filters backwash water of IWTP.
Results: Survival of E.coli O157:H7 in sludge sample of sedimentation basin was indicated by formation of agglutination particles in immunological method and through indicator probes in the RT-PCR method. However, E.coli O157:H7 was not detected in water samples of other units of IWTP. The removal percent of TC, FC, and HPC were: 59.5, 49, and 54.8 % in sedimentation basin 66, 45.8, and 57 % in ozonation: 98.8, 98, and 78.8 in the filtration and 96, 100, 91% in disinfection, respectively.
Conclusion: This study approved the existence of the pathogenic coliform, E.coli O157:H7 in the
sludge of sedimentation basin. Absent of E.coli O157:H7 in the finished water indicates that the existing units of IWTP could eliminate these pathogenic bacteria, before reaching the final units of the plant, including the filters and disinfection.

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Type of Study: Research | Subject: General
Received: 2010/08/10 | Accepted: 2010/11/3 | Published: 2013/07/8

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