Iranian Journal of Health and Environment
سلامت و محیط زیست
ijhe
Medical Sciences
http://ijhe.tums.ac.ir
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بررسی شیوع استافیلوکوکوس اورئوس و الگوی مقاومت آنتی بیوتیکی آن در پنیرهای سنتی گوسفندی چالوس (استان مازندران، ایران) در سال ۱۴۰۰
An investigation on the prevalence and antibiotic resistance pattern of Staphylococcus aureus in traditional sheep cheese from Chalous (Mazandaran province, Iran) in 2021-2022
عمومى
General
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">زمینه و هدف: </span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">یکی از دلایل<b> </b>آلودگی پنیر به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> <i>استافیلوکوکوس اورئوس</i></span></span></span> <span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">)</span></span><i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Staphylococcus aureus; S.aureus</span></span></i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">(</span></span><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">استفاده از شیر غیرپاستوریزه و روشهای نادرست فرآوری پنیر است. با توجه به </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" lotus="" style="font-family:"><span style="color:black">اهمیت باکتری </span></span></span></span><i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">S.aureus</span></span></i> <span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" lotus="" style="font-family:"><span style="color:black">در ایجاد مسمومیتها و تاکید سازمان جهانی بهداشت بر پایش مستمر</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="background:white"><span b="" lotus="" style="font-family:"><span style="color:black">این </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" lotus="" style="font-family:"><span style="color:black">باکتری</span></span></span></span><i><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">، </span></span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">در این مطالعه مقطعی، شیوع </span></span></span> <i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">S.aureus</span></span></i><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> و الگوی مقاومت آنتی بیوتیکی آن در پنیرهای سنتی گوسفندی چالوس (استان مازندران، ایران) در سال ۱۴۰۰ مورد ارزیابی قرار گرفت.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">روش بررسی:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> تعداد ۴۵ نمونه پنیر سنتی گوسفندی تهیه شده از فروشگاههای لبنیات سنتی چالوس </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">(استان مازندران، ایران)</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> به صورت تصادفی جمع</span></span></span><span dir="LTR" lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">‎</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">آوری شدند. نمونه ها در ابتدا از نظر آلودگی با </span></span></span> <i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">S.aureus</span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">با روش کشت، ارزیابی شدند. در نهایت الگوی مقاومت آنتی</span></span></span><span dir="LTR" lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">‎</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">بیوتیکی جدایه</span></span></span><span dir="LTR" lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">‎</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">ها نسبت به ۹ آنتی</span></span></span><span dir="LTR" lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">‎</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">بیوتیک مختلف با استفاده از روش انتشار دیسکی مورد بررسی قرار گرفت.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">یافتهها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> از تعداد ۴۵ نمونه پنیرهای سنتی گوسفندی جمع</span></span></span><span dir="LTR" lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">‎</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">آوری شده، ۱۷ مورد (37/8 درصد) از نمونه</span></span></span><span dir="LTR" lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">‎</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">ها آلوده به </span></span></span><i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">S.aureus</span></span></i><i> </i><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">بودند. از مجموع ۱۸ جدایه به دست آمده از نمونه های پنیر سنتی چالوس، بیشترین مقاومت به پنیسیلین (64/7 درصد) و تتراسایکلین (58/8 درصد) مربوط بود، در حالی که کمترین مقاومت در برابر جنتامایسین و اریترومایسین (23/5 درصد) مشاهده شد. همچنین ۱۷ جدایه به دست آمده از نمونه</span></span></span><span dir="LTR" lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">‎</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">های پنیر سنتی گوسفندی نسبت به ۲ تا ۸ آنتی بیوتیک مقاومت همزمان نشان دادند.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">نتیجه</span></span></span></b><b><span dir="LTR" lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">‎</span></span></span></b><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">گیری:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> میزان بالای آلودگی به </span></span></span> <i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">S.aureus</span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">در پنیرهای سنتی گوسفندی تهیه شده در چالوس (مازندران، ایران) مشاهده شد. با رعایت اصول بهداشتی در دامداریها و آموزش بهداشت عمومی جهت جلوگیری از بروز آلودگیهای ثانویه، می توان از شیوع گسترده این باکتری در محصولات سنتی پیشگیری نمود.</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span><br>
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<div style="text-align: justify;"><span style="font-size:11pt"><span style="background:white"><span style="line-height:106%"><span calibri="" style="font-family:"><b><span style="line-height:106%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Background and Objective</span></span></span></b><b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:106%"><span b="" lotus="" style="font-family:"><span style="color:black">:</span></span></span></span></b><b> </b><span style="background:white"><span style="line-height:106%"><span new="" roman="" style="font-family:" times=""><span style="color:black">One of the main causes of cheese contamination with <em>S.aureus</em> is the use of unpasteurized milk. Improper cheese processing methods can also lead to contamination with this bacterium. Considering the significant role of <i>S.<em>aureus</em></i> in foodborne intoxications and the World Health Organization’s recommendation for continuous surveillance of this bacterium, this cross-sectional study </span></span></span></span><span lang="EN-GB" style="background:white"><span style="line-height:106%"><span new="" roman="" style="font-family:" times=""><span style="color:black">aims </span></span></span></span><span style="background:white"><span style="line-height:106%"><span new="" roman="" style="font-family:" times=""><span style="color:black">to determine the prevalence and antibiotic resistance pattern of <em>S.aureus</em> in traditional sheep cheese from</span></span></span></span> <span style="background:white"><span style="line-height:106%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Chalous (Mazandaran Province, Iran).</span></span></span></span><span lang="EN-GB" style="line-height:106%"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="line-height:106%"><span calibri="" style="font-family:"><b><span style="line-height:106%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Materials and Methods: </span></span></span></b><span lang="EN-GB" style="line-height:106%"><span new="" roman="" style="font-family:" times=""><span style="color:black">A total of 45 samples of traditional sheep cheese were randomly collected from local dairy shops in Chalous. Initially, the samples were examined for contamination with <i>S.aureus</i> using culture-based methods. Finally, the antibiotic resistance pattern of the isolates against nine different antibiotics were determined using the disk diffusion method.</span></span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:106%"><span b="" lotus="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="line-height:106%"><span calibri="" style="font-family:"><strong><span style="line-height:106%"><span style="color:black">Results:</span></span></strong><span style="line-height:106%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> Among 45 collected samples of traditional sheep cheese, 17 samples (37.8%) were contaminated with <em>S. aureus</em>. Of the 18 isolates obtained from traditional Chalus cheese samples, the highest resistance was observed against penicillin (64.7%) and tetracycline (58.8%), whereas the lowest resistance was detected against gentamicin and erythromycin (23.5%). Moreover, the 17 isolates obtained from traditional sheep cheese samples exhibited simultaneous resistance to 2 to 8 antibiotics.</span></span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:106%"><span b="" lotus="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="line-height:106%"><span calibri="" style="font-family:"><b><span style="line-height:106%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Conclusion: </span></span></span></b><span style="background:white"><span style="line-height:106%"><span new="" roman="" style="font-family:" times=""><span style="color:black">A high level of contamination with <em>S.aureus</em> in traditional sheep cheeses produced in Chalous (Mazandaran, Iran), was observed. Adherence to hygienic practices in dairy farms and the implementation of public health education can help prevent secondary contamination during cheese processing and reduce the widespread dissemination of this bacterium in traditional dairy products.</span></span></span></span><span lang="EN-GB" style="line-height:106%"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span><br>
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استافیلوکوکوس اورئوس, پنیر سنتی گوسفندی, چالوس
S.aureus, Traditional sheep cheese, Chalous
669
684
http://ijhe.tums.ac.ir/browse.php?a_code=A-10-3028-1&slc_lang=fa&sid=1
Hiwa
Karimi Darreh Abi
هیوا
کریمی دره آبی
hiva60iran@yahoo.com