Volume 3, Number 3 (4 2010)                   ijhe 2010, 3(3): 0-0 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Atabakhsh P, Amin M, Mortazavi H, Yaran M, Akhavan Sepahi A, Nouhi A et al . Identification of Total and Fecal Coliforms and Heterotrophic to Microbiological Method and E.coli O157:H7 to Immunological, and RealTime PCR Methodsin IsfahanWaterTreatment Plant. ijhe. 2010; 3 (3)
URL: http://ijhe.tums.ac.ir/article-1-115-en.html

Abstract:   (6245 Views)

Backgrounds and Objectives:Total and Fecal coliforms (TC and FC), heterotrophic plate count (HPC), were counted by microbiological method and E.coli O157:H7 were detected through immunological and Real time PCRmethods inwater intake and all of units of Isfahanwater treatment plant (IWTP).
Materials and Methods: The microbial profile including TC, FC, and HPC, were monitored and turbidity and total organic carbon were analyzed in 8 locations of water intake, and unit operation and processes of IWTP, including, inlet, sedimentation, ozonation, and filtration and finished water. Immunological method through anti-serum kits and molecular method of RT-PCR were used to detect E.coli O157:H7 in the 8 locations and also the sludge of the sedimentation basin and filters backwash water of IWTP.
Results: Survival of E.coli O157:H7 in sludge sample of sedimentation basin was indicated by formation of agglutination particles in immunological method and through indicator probes in the RT-PCR method. However, E.coli O157:H7 was not detected in water samples of other units of IWTP. The removal percent of TC, FC, and HPC were: 59.5, 49, and 54.8 % in sedimentation basin 66, 45.8, and 57 % in ozonation: 98.8, 98, and 78.8 in the filtration and 96, 100, 91% in disinfection, respectively.
Conclusion: This study approved the existence of the pathogenic coliform, E.coli O157:H7 in the
sludge of sedimentation basin. Absent of E.coli O157:H7 in the finished water indicates that the existing units of IWTP could eliminate these pathogenic bacteria, before reaching the final units of the plant, including the filters and disinfection.

Full-Text [PDF 1182 kb]   (1655 Downloads)    
Type of Study: Research | Subject: General
Received: 2010/08/10 | Accepted: 2010/11/3 | Published: 2013/07/8

Add your comments about this article : Your username or email:
Write the security code in the box

© 2017 All Rights Reserved | Iranian Journal of Health and Environment

Designed & Developed by : Yektaweb